AKTIVITAS HIPOGLIKEMI DARI TEH HERBAL CAMPURAN HERBA SAMBILOTO (Andrographis Paniculata) DAN BIJI MAHONI (Swietenia Mahagoni) DAN PROFIL METABOLIT SEKUNDER TAHUN 2022
Keywords:
Andrographis paniculata, Swietenia mahagoni, hypoglycemic activity, herbal teaAbstract
Diabetes mellitus is a chronic metabolic disorder that continues to present a major world wide health problem. Numerous herbal drugs like Andrographis paniculata and Swietenia mahagoni have been used by people of various cultures to treat diabetes. The aim of this research was to measure the hypoglycemic activity herbal tea of Andrographis paniculata herbs and Swietenia mahagoni seeds and their combination in alloxan- induced diabetic mice. The tea was prepared by brewing 10 g herbal tea with 100 ml of boiling water for 10 minutes. The herbal tea of Andrographis paniculata herbs, Swietenia mahagoni seeds, combination with ratio 1:1, 1:2 and 2:1 (0.4 ml/20 g BW), was administered orally to groups I, II, III, IV and V respectively. The reference drug glibenclamide (0,013 mg/10 g BW) and CMC-Na were also administered orally to animals in positive and negative control group respectively.
Oral administration of herbal tea for seven days resulted in a slight reduction in blood glucose level. Statistically there is no groups that has significant difference with negative control groups (P < 0.05) except positive control. Therefore the herbal tea combination of Andrographis paniculata herbs and Swietenia mahagoni seeds with ratio 2:1, showed the biggest reduction in blood glucose level (88.20 ± 43.16 mg/dl). The secondary metabolite profiling was done by establish the TLC and chromatogram profile. TLC profiling was performed out using Chloroform:Methanol (9:1) as mobile phase. Then, TLC plate scanned in wavelength 254 nm and 366 nm using CAMAG TLC Scanner 3.
Downloads
References
Akbar, S., 2011. Andrographis paniculata: A Review of Pharmacological Activities and Clinical Effects. Alternative Medicine Review, Vol. 16, No. 1, p. 66-77.
Debasis, D., Kausik, C., Kazi, M.A., Tushar, K.B., and Debidas, G., 2011. Antidiabetic Potentiality of the Aqueous-Methanolic Extract of Seed of Swietenia mahagoni (L.) Jacq. In Streptozotocin-Induced Diabetic Male Albino Rat: A Correlative and Evidence-Based Approach with Antioxidative and Antihyperlipidemic Activities. Evidence-Based Complementary and Alternative Medicine, Vol. 2011.
Departemen Kesehatan Republik Indonesia., 2008. Riset Kesehatan Dasar Tahun 2007. Jakarta
Departemen Kesehatan Republik Indonesia., 2008. Farmakope Herbal Indonesia. Jakarta.
Dipiro, J.T., Talbert, R.L., Yee, G.C., Matzke, G.R., Wells, B.G., and Posey, L.M., 2008. Pharmacotherapy: A Patophysiologic Approach, 7th Edition, New York: Mc Graw Hill.
EMEA (European Medicines Agency)., 2006. Guideline on specifications: Test procedures and acceptance criteria for herbal substances, herbal preparations and herbal medicinal products/traditional herbal medicinal products. London.
Etuk, E.U., 2010. Animals Models for Studying Diabetes Mellitus. Agriculture and Biology Journal of North America, Vol. 1(2), p. 130-134.
Fatmawati, E., 2008. Pengaruh Lama Pemberian Ekstrak Daun Sambiloto (Andrographis paniculata Ness.) Terhadap Kadar Kolesterol, LDL (Low Density Lipoprotein), HDL (High Density Lipoprotein) dan Trigliserida Darah Tikus (Rattus norvegicus) Diabetes. Malang: Universitas Islam Negeri.
Fried, B., and Sherma, J., 2005. Thin-layer chromatography. New york: Taylor and Francis e-Library.
Handa, S.S., Khanuja, S.P.S., Longo, G., Rakesh, D.D., 2008. Extraction Technologies for Medicinal and Aromatic Plants. International Centre for Science and High Technology, Trieste.
Hossain, M.D.A., Roy, B.K., Ahmed, K., Chowdhury, A.M.S., and Rashid, M.A., 2007. Antidiabetic Activity of Andrographis paniculata. Dhaka Univ. J. Pharm. Sci, Vol. 6(1), p. 15-20.
Kennedy, D.O., and Wightman, E.L., 2011. Herbal Extracts and Phytochemicals: Plant Secondary Metabolites and the Enhancement of Human Brain Function. Advances in Nutrition: An International Review Journal, Vol. 2, p. 32-50.
Kumar, P.R., Sujatha, D., Saleem, M.T.S., Chetty, M.C., and Ranganayakulu, D., 2010. Potential antidiabetic and antioxidant activities of Morus indica and Asystasia gangetica in alloxan induced diabetes mellitus. Journal of Experimental Pharmacology, Vol. 2, p. 29-36.
Laurence, D.R. and Bacharach, A.L., 1964. Evaluation of Drug Activities: Pharmacometrics. London and New York: Academic Press.
Lenzen, S., 2008. The Mechanism of Alloxan and Streptozotocin Induced Diabetes. Diabetologia 51. p. 216-226.
Li, D., Chen, J., Chen, Q., Li, G., Chen, J., Yue, J.,Chen, M., Wang, X., Shen, J.,Shen, X., and Jiang, H., 2005. Swietenia Mahagony Extract Shows Agonistic Activity to PPARγ and Gives Ameliorative Effects on Diabetic Mice. Acta Pharmacologia Sinica Vol.(2) p. 220–222
Lwanga, S.K. and Lemeshow, S., 1998. Sample Size Determination in Health Studies. World Health Organization: Geneva.
Orwa, C., Mutua, A., Kindt, R., Jamnadass, R., and Anthony, S., 2009. Agroforestree Database: A Tree Reference and Selection Guide Version 4.0. Kenya: World Agroforestry Centre.
Pasaribu, E., 2011. Isolasi Senyawa Terpenoida dari Kulit Buah Mahoni ( Swietenia mahagoni (L.) Jacq. ). Medan: Universitas Sumatera Utara.
Patil, S.S., and Bonde, C.G., 2009. Development and Validation of Analitycal Method for Simultaneous Estimation of Glibenclamide and Metformin Hcl in Bulk and Tablets using UV-Visible Spectroscopy. International Journal of ChemTech Research, Vol. 1, No. 4, pp. 905-909.
Preedy, V.R., Watson, R.R., and Patel, V.B., 2011. Nuts and Seeds in Health and Disease Prevention. United Kingdom: Academic Press.
Pujiasmanto, B., Moenandir, J., Syamsulbahri, dan Kuswanto, 2007. Kajian Agroekologi dan Morfologi Sambiloto (Andrographis paniculata Ness.) pada Berbagai Habitat. Biodiversitas, Vol. 8, No. 4, hal. 326- 329.
Raja, L.L., 2008. Uji Efek Ekstrak Etanol Biji Mahoni (Swietenia mahagoni Jacq) Terhadap Penurunan Kadar Gula Darah Tikus Putih. Medan: Universitas Sumatera Utara.
Rao, N.K., 2006. Anti-Hyperglycemic and Renal Protective Activities of Andrographis paniculata Roots Chloroform Extract. Iranian Journal of Pharmacology and Therapeutics, Vol. 5, No. 1, p. 47- 50.
Rao, P.A., and Jamil, K., 2011. Pharmacological Evaluation of Herbal Extracts for Their Invitro Hypoglycemic Activity. International Journal of Phytopharmacology, Vol. 2, No. 1, p. 15-21.
Rathnakar, U.P., Hashim, S.K., Pemminati, S., D.Ullal, S., Pai, M.R.S.M., Nandita, V., Farsana U.K., Aboobecker, S.P.A., 2010. Hypoglycemic Effect of YXK12 (polyherbal product) in Normal Rats. Journal of Pharmacy Research 2010 Vol.3 (7) : 1517-1518
Reyes, B.A.S., Bautista, N.D., Tanquilut, N.C., Anunciado, R.V., Leung, A.B., Sanchez, G.C., Magtoto, R.L., Castronuevo, P., Tsukamura, H., and Maeda, K.I., 2006. Anti-Diabetic Potentials of Momordica charantia and Andrographis paniculata and Their Effects on Estrous Cyclicity of Alloxan-Induced Diabetic Rats. Journal of Ethnopharmacology, Vol. 105, Issues 1–2, p. 196–200.
Said, O., Fulder, S., Khalil, K., Azaizeh, H., Kassis, E., and Saad, B., 2007. Maintaining A Physiological Blood Glucose Level with
‘Glucolevel’, A Combination of Four Anti-Diabetes Plants Used in the Traditional Arab Herbal Medicine. eCAM, Vol. 5, No. 4, p. 421– 428.
Saleh, C., 2007. Isolasi dan Penentuan Struktur Senyawa Steroid Dari Akar Tumbuhan Cendana (Santalum album Linn). Medan: Universitas Sumatera Utara.
Sharma, A., 2012. Transdermal Approach of Antidiabetic Drug Glibenclamide: A Review. International Journal of Pharmaceutical Research and Development, Vol. 3(11), p. 25-32.
Suherman, K.H. dan Nafrialdi, 2011. Insulin dan Antidiabetik Oral. Di dalam buku Farmakologi dan Terapan edisi 5. Jakarta : Balai penerbit FKUI
Sulistyono, E., 2011. Efek Penurunan Kadar Glukosa Darah Infusa Biji
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Hasna Dewi
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
